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pan dendritic cell dc isolation kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec pan dendritic cell dc isolation kit
    Pan Dendritic Cell Dc Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pan+dc+isolation+kit/Pan+Dendritic+Cell+Isolation+Kit%2C+mouse/us12636317-760-8-14
    Average 95 stars, based on 115 article reviews
    pan dendritic cell dc isolation kit - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Suspension:

    Article Title: Syndromic immune disorder caused by a viable hypomorphic allele of spliceosome component Snrnp40
    Article Snippet: .. Seven days later, the suspension cells and loosely attached cells were collected and purified for BMDCs using the Pan DC Isolation Kit (Miltenyi Biotec). ..

    Purification:

    Article Title: Syndromic immune disorder caused by a viable hypomorphic allele of spliceosome component Snrnp40
    Article Snippet: .. Seven days later, the suspension cells and loosely attached cells were collected and purified for BMDCs using the Pan DC Isolation Kit (Miltenyi Biotec). ..

    Article Title: Tolerogenic nanoparticles suppress central nervous system inflammation
    Article Snippet: .. Splenic mouse DCs were purified from naive B6 mice using the pan DC isolation kit according to the manufacturer’s instructions (Miltenyi). .. Human DCs were purified from healthy donor leukopaks purchased from Research Blood Components using the Blood Dendritic Cell Isolation Kit II following the manufacturer’s instructions (Miltenyi).

    Article Title: Immunological response of using emulsifying elongation factor 1 alpha of Plasmodium falciparum-based vaccines in complete Freund's adjuvant.
    Article Snippet: DCs were enriched by a nonionic iodinated gradient media with 17.5% HistodenzTM (Sigma-Aldrich). .. Harvested myeloid cells were purified by magnetic sorting (MACS) using the Pan DC isolation kit, mouse (Miltenyi Biotec). .. The immature DCs were activated with 100 ng/mL Lipopolysaccharides from Escherichia coli O26:B6 (LPS) (Sigma-Aldrich) in RPMI 1640 media containing 10% heat-inactivated FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, 50 μM beta-mercapto, and 1 mM sodium-pyruvate (complete media) and pulsed with 10 μg/mL relevant or irrelevant antigen for 24 h. To co-culture antigenic stimulated splenic DCs with splenic T cells, three of immunized mice were sacrificed and spleens were excised.

    Isolation:

    Article Title: Syndromic immune disorder caused by a viable hypomorphic allele of spliceosome component Snrnp40
    Article Snippet: .. Seven days later, the suspension cells and loosely attached cells were collected and purified for BMDCs using the Pan DC Isolation Kit (Miltenyi Biotec). ..

    Article Title: Tolerogenic nanoparticles suppress central nervous system inflammation
    Article Snippet: .. Splenic mouse DCs were purified from naive B6 mice using the pan DC isolation kit according to the manufacturer’s instructions (Miltenyi). .. Human DCs were purified from healthy donor leukopaks purchased from Research Blood Components using the Blood Dendritic Cell Isolation Kit II following the manufacturer’s instructions (Miltenyi).

    Article Title: Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia
    Article Snippet: .. Blood was collected by cardiac puncture for plasma isolation and splenic dendritic cells ( DCs ) were isolated using a Pan DC Isolation Kit (Miltenyi Biotec; 130-100-875). ..

    Article Title: Immunological response of using emulsifying elongation factor 1 alpha of Plasmodium falciparum-based vaccines in complete Freund's adjuvant.
    Article Snippet: DCs were enriched by a nonionic iodinated gradient media with 17.5% HistodenzTM (Sigma-Aldrich). .. Harvested myeloid cells were purified by magnetic sorting (MACS) using the Pan DC isolation kit, mouse (Miltenyi Biotec). .. The immature DCs were activated with 100 ng/mL Lipopolysaccharides from Escherichia coli O26:B6 (LPS) (Sigma-Aldrich) in RPMI 1640 media containing 10% heat-inactivated FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, 50 μM beta-mercapto, and 1 mM sodium-pyruvate (complete media) and pulsed with 10 μg/mL relevant or irrelevant antigen for 24 h. To co-culture antigenic stimulated splenic DCs with splenic T cells, three of immunized mice were sacrificed and spleens were excised.

    Article Title: Semaphorin 3A contributes to sepsis-induced immunosuppression by impairing CD4 + T cell anergy
    Article Snippet: Spleens were harvested and prepared as single-cell suspensions and then subjected to Ficoll-Paque density gradient centrifugation (cat. no. LTS1092P; Tianjin TBD Science) at 4°C, 1,500 × g for 15 min. Lymphocytes [CD4 + T cells, CD4 + CD25 + regulatory T cells (Tregs)], B cells and natural killer (NK) cells were isolated from mononuclear cells using a CD4 + T cell Isolation kit, mouse (cat. no. 130-104-454), a CD4 + CD25 + regulatory T cell Isolation kit, mouse (cat. no. 130-091-041), a Pan B Cell Isolation kit II, mouse (cat. no. 130-104-443), and a NK Cell Isolation kit, mouse (cat. no. 130-115-818; all from Miltenyi Biotec GmbH) and autoMACS ® Columns (Miltenyi Biotec GmbH) according to the manufacturer's instructions. .. Cells of the myeloid lineage, including macrophage (Mø) cells and dendritic cells (DC), were isolated from the abdominal cavity and femur, respectively, using a Mø Isolation kit (Peritoneum), mouse (cat. no. 130-110-434) and a Pan DC Isolation kit, mouse (cat. no. 130-100-875; both from Miltenyi Biotec GmbH) and autoMACS ® Columns (Miltenyi Biotec GmbH) according to the manufacturer's instructions. .. Isolated cells were cultured in RPMI-1640 medium (Nanjing KeyGen Biotech Co., Ltd.) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich; Merck KGaA) and incubated at 37°C with 5% CO 2 , following treatment with anti-CD3e (5 μg/ml; cat. no. 564378; BD Pharmingen; BD Biosciences) and anti-CD28 (2 μg/ml; cat. no. 562767; BD Pharmingen; BD Biosciences) antibodies for polyclonal activation of T cells.

    Article Title: Inhibitor of Apoptosis Proteins Antagonist Induces T-cell Proliferation after Cross-Presentation by Dendritic Cells
    Article Snippet: .. Pan DCs (both classical DCs and plasmacytoid DCs) were isolated from spleens fromCas9-EGFP orWTC57BL/6JRj mice using Pan DC Isolation Kit (Miltenyi Biotec, 130–100–875). .. Briefly, spleens were mashed through a 70-mm filter (BD Biosciences) and incubated in erylysis buffer (BioLegend, 420301) on ice for 7 minutes.

    Article Title: CD4⁺ T cells confer transplantable rejuvenation via Rivers of telomeres
    Article Snippet: .. Alternatively, CD11c+ dendritic cells (DCs) were isolated from spleens of young C57BL/6 mice using the Pan-DC isolation kit (Miltenyi, 130-100-875). .. DCs were pulsed with OVA peptide (OT-II 323–339, 1 μg/ml; InvivoGen) for 3 h at 37 °C before transfer into OT-II recipient mice, with or without prior CD4+ T cell depletion.

    Clinical Proteomics:

    Article Title: Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia
    Article Snippet: .. Blood was collected by cardiac puncture for plasma isolation and splenic dendritic cells ( DCs ) were isolated using a Pan DC Isolation Kit (Miltenyi Biotec; 130-100-875). ..

    Magnetic Cell Separation:

    Article Title: Immunological response of using emulsifying elongation factor 1 alpha of Plasmodium falciparum-based vaccines in complete Freund's adjuvant.
    Article Snippet: DCs were enriched by a nonionic iodinated gradient media with 17.5% HistodenzTM (Sigma-Aldrich). .. Harvested myeloid cells were purified by magnetic sorting (MACS) using the Pan DC isolation kit, mouse (Miltenyi Biotec). .. The immature DCs were activated with 100 ng/mL Lipopolysaccharides from Escherichia coli O26:B6 (LPS) (Sigma-Aldrich) in RPMI 1640 media containing 10% heat-inactivated FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, 50 μM beta-mercapto, and 1 mM sodium-pyruvate (complete media) and pulsed with 10 μg/mL relevant or irrelevant antigen for 24 h. To co-culture antigenic stimulated splenic DCs with splenic T cells, three of immunized mice were sacrificed and spleens were excised.



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